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rabbit polyclonal anti-vasoactive intestinal peptide (vip  (ImmunoStar inc)

 
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    Structured Review

    ImmunoStar inc rabbit polyclonal anti-vasoactive intestinal peptide (vip

    Rabbit Polyclonal Anti Vasoactive Intestinal Peptide (Vip, supplied by ImmunoStar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti-vasoactive+intestinal+peptide+(vip/rabbit+anti+vasoactive+intestinal+peptide/pmc10831933-333-67-74
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti-vasoactive intestinal peptide (vip - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Ventral hippocampal cholecystokinin interneurons gate contextual reward memory"

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory

    Journal: iScience

    doi: 10.1016/j.isci.2024.108824


    Figure Legend Snippet:

    Techniques Used: Virus, Plasmid Preparation, Recombinant, Software

    Related Articles

    Immunostaining:

    Article Title: Synaptic Specializations of Melanopsin-Retinal Ganglion Cells in Multiple Brain Regions Revealed by Genetic Label for Light and Electron Microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-miniSOG A gift from Roger Tsien’s laboratory N/A Rabbit polyclonal anti-VIP Immunostar Cat# 20077; RRID: AB_572270 FluoroMyelin Thermo Fisher Scientific Cat# F34652; RRID: AB_2572213 Bacterial and Virus Strains AAV2-EF1a-DIO-miniSOG-f The Salk vector core N/A AAV2-EF1a-DIO-tdTomato-f The Salk vector core N/A Chemicals, Peptides, and Recombinant Proteins Normal donkey serum Jackson ImmunoResearch Cat# 017-000-001 Glutaraldehyde Electron Microscopy Sciences Cat# 16200 Paraformaldehyde Electron Microscopy Sciences Cat# 19200 Glycine Electron Microscopy Sciences Cat# 16200 Potassium cyanide Electron Microscopy Sciences Cat# 19200 Aminotriazole Sigma-Aldrich Cat# G7126 3,30-Diaminobenzidine Free Base Sigma-Aldrich Cat# D8801 Potassium ferrocyanide BAKER ANALYZED A.C.S.

    Article Title: Immunohistochemical Distribution and Neurochemical Characterization of Huntingtin-Associated Protein 1 Immunoreactive Neurons in the Adult Mouse Lingual Ganglia
    Article Snippet: Rabbit Polyclonal anti-VIP , Porcine VIP coupled to bovine thyroglobulin with carbodiimide , Cat# 20077, RRID: AB_572270 , Immunostar, Hudson, WI, USA , 1:5000 , [ ] .

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Fluorophore-conjugated secondary antibodies were from Life Technologies (Alexa Fluor 568 goat anti-rat #A11077, Alexa Fluor 488 donkey anti-mouse #A21202, Alexa Fluor 647 donkey anti-goat #A21447) and Jackson ImmunoResearch (Cy2 donkey anti-goat #705-225-147, Cy2 donkey anti-guinea pig #706-225-148, Cy3 donkey anti-rabbit #711-165-152, Cy3 donkey anti-mouse #715-165-151, Cy3 donkey anti-goat #705-165-147, Alexa Fluor 488 donkey anti-guinea pig #706-545-148, Cy5 donkey anti-mouse #715-175-511, Cy5 donkey anti-rabbit #711-175-152, Alexa Fluor 647 donkey anti-guinea pig #706-605-148).

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Rabbit polyclonal anti-VIP , Immunostar , Cat# 20077; RRID: AB_572270; LOT# 1513001.

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: For immunostaining, free-floating brain sections were blocked with 5% normal donkey serum in 0.1% Triton X-100 in PBS (PBS-T) for 2 h. Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK-8 (1:1000, Sigma Millipore, C2581, rabbit anti-proCCK (Frontier Institute, Af350), rabbit polyclonal anti-GABA (1:1000, Sigma Millipore, A2052), rabbit polyclonal anti-PV antibody (1:1000, Abcam, ab11427), rabbit polyclonal anti-vasoactive intestinal peptide (VIP) (1:500, Immunostar, 20077), rat polyclonal anti-somatostatin (1:500, Millipore, MAB354), rabbit polyclonal anti-cFos (1:1000, Santa Cruz Biotechnology), chicken polyclonal anti-GFP (1:1000, Abcam, ab13970), and goat polyclonal anti-mCherry (1:1000, Sicgen, AB0040-200).

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: Rabbit polyclonal anti-vasoactive intestinal peptide , Immunostar , Cat# 20077; RRID: AB_572270.

    Article Title: Cholecystokinin-Expressing Interneurons of the Medial Prefrontal Cortex Mediate Working Memory Retrieval
    Article Snippet: Primary antibody incubation was followed by incubation for 2 h at room temperature with PBS-T containing the following secondary antibodies: AlexaFluor-488-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711545152), AlexaFluor-594-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 715515152), Cy5-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711175152), AlexaFluor-488-conjugated donkey anti-chicken (1:1000, Jackson ImmunoResearch Laboratories, 703545145), and AlexaFluor-594-conjugated donkey anti-goat (1:1000, Jackson ImmunoResearch Laboratories, 705515147).

    Incubation:

    Article Title: Synaptic Specializations of Melanopsin-Retinal Ganglion Cells in Multiple Brain Regions Revealed by Genetic Label for Light and Electron Microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-miniSOG A gift from Roger Tsien’s laboratory N/A Rabbit polyclonal anti-VIP Immunostar Cat# 20077; RRID: AB_572270 FluoroMyelin Thermo Fisher Scientific Cat# F34652; RRID: AB_2572213 Bacterial and Virus Strains AAV2-EF1a-DIO-miniSOG-f The Salk vector core N/A AAV2-EF1a-DIO-tdTomato-f The Salk vector core N/A Chemicals, Peptides, and Recombinant Proteins Normal donkey serum Jackson ImmunoResearch Cat# 017-000-001 Glutaraldehyde Electron Microscopy Sciences Cat# 16200 Paraformaldehyde Electron Microscopy Sciences Cat# 19200 Glycine Electron Microscopy Sciences Cat# 16200 Potassium cyanide Electron Microscopy Sciences Cat# 19200 Aminotriazole Sigma-Aldrich Cat# G7126 3,30-Diaminobenzidine Free Base Sigma-Aldrich Cat# D8801 Potassium ferrocyanide BAKER ANALYZED A.C.S.

    Article Title: Immunohistochemical Distribution and Neurochemical Characterization of Huntingtin-Associated Protein 1 Immunoreactive Neurons in the Adult Mouse Lingual Ganglia
    Article Snippet: Rabbit Polyclonal anti-VIP , Porcine VIP coupled to bovine thyroglobulin with carbodiimide , Cat# 20077, RRID: AB_572270 , Immunostar, Hudson, WI, USA , 1:5000 , [ ] .

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Fluorophore-conjugated secondary antibodies were from Life Technologies (Alexa Fluor 568 goat anti-rat #A11077, Alexa Fluor 488 donkey anti-mouse #A21202, Alexa Fluor 647 donkey anti-goat #A21447) and Jackson ImmunoResearch (Cy2 donkey anti-goat #705-225-147, Cy2 donkey anti-guinea pig #706-225-148, Cy3 donkey anti-rabbit #711-165-152, Cy3 donkey anti-mouse #715-165-151, Cy3 donkey anti-goat #705-165-147, Alexa Fluor 488 donkey anti-guinea pig #706-545-148, Cy5 donkey anti-mouse #715-175-511, Cy5 donkey anti-rabbit #711-175-152, Alexa Fluor 647 donkey anti-guinea pig #706-605-148).

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Rabbit polyclonal anti-VIP , Immunostar , Cat# 20077; RRID: AB_572270; LOT# 1513001.

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: For immunostaining, free-floating brain sections were blocked with 5% normal donkey serum in 0.1% Triton X-100 in PBS (PBS-T) for 2 h. Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK-8 (1:1000, Sigma Millipore, C2581, rabbit anti-proCCK (Frontier Institute, Af350), rabbit polyclonal anti-GABA (1:1000, Sigma Millipore, A2052), rabbit polyclonal anti-PV antibody (1:1000, Abcam, ab11427), rabbit polyclonal anti-vasoactive intestinal peptide (VIP) (1:500, Immunostar, 20077), rat polyclonal anti-somatostatin (1:500, Millipore, MAB354), rabbit polyclonal anti-cFos (1:1000, Santa Cruz Biotechnology), chicken polyclonal anti-GFP (1:1000, Abcam, ab13970), and goat polyclonal anti-mCherry (1:1000, Sicgen, AB0040-200).

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: Rabbit polyclonal anti-vasoactive intestinal peptide , Immunostar , Cat# 20077; RRID: AB_572270.

    Article Title: Cholecystokinin-Expressing Interneurons of the Medial Prefrontal Cortex Mediate Working Memory Retrieval
    Article Snippet: Primary antibody incubation was followed by incubation for 2 h at room temperature with PBS-T containing the following secondary antibodies: AlexaFluor-488-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711545152), AlexaFluor-594-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 715515152), Cy5-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711175152), AlexaFluor-488-conjugated donkey anti-chicken (1:1000, Jackson ImmunoResearch Laboratories, 703545145), and AlexaFluor-594-conjugated donkey anti-goat (1:1000, Jackson ImmunoResearch Laboratories, 705515147).

    Virus:

    Article Title: Synaptic Specializations of Melanopsin-Retinal Ganglion Cells in Multiple Brain Regions Revealed by Genetic Label for Light and Electron Microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-miniSOG A gift from Roger Tsien’s laboratory N/A Rabbit polyclonal anti-VIP Immunostar Cat# 20077; RRID: AB_572270 FluoroMyelin Thermo Fisher Scientific Cat# F34652; RRID: AB_2572213 Bacterial and Virus Strains AAV2-EF1a-DIO-miniSOG-f The Salk vector core N/A AAV2-EF1a-DIO-tdTomato-f The Salk vector core N/A Chemicals, Peptides, and Recombinant Proteins Normal donkey serum Jackson ImmunoResearch Cat# 017-000-001 Glutaraldehyde Electron Microscopy Sciences Cat# 16200 Paraformaldehyde Electron Microscopy Sciences Cat# 19200 Glycine Electron Microscopy Sciences Cat# 16200 Potassium cyanide Electron Microscopy Sciences Cat# 19200 Aminotriazole Sigma-Aldrich Cat# G7126 3,30-Diaminobenzidine Free Base Sigma-Aldrich Cat# D8801 Potassium ferrocyanide BAKER ANALYZED A.C.S.

    Article Title: Immunohistochemical Distribution and Neurochemical Characterization of Huntingtin-Associated Protein 1 Immunoreactive Neurons in the Adult Mouse Lingual Ganglia
    Article Snippet: Rabbit Polyclonal anti-VIP , Porcine VIP coupled to bovine thyroglobulin with carbodiimide , Cat# 20077, RRID: AB_572270 , Immunostar, Hudson, WI, USA , 1:5000 , [ ] .

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Fluorophore-conjugated secondary antibodies were from Life Technologies (Alexa Fluor 568 goat anti-rat #A11077, Alexa Fluor 488 donkey anti-mouse #A21202, Alexa Fluor 647 donkey anti-goat #A21447) and Jackson ImmunoResearch (Cy2 donkey anti-goat #705-225-147, Cy2 donkey anti-guinea pig #706-225-148, Cy3 donkey anti-rabbit #711-165-152, Cy3 donkey anti-mouse #715-165-151, Cy3 donkey anti-goat #705-165-147, Alexa Fluor 488 donkey anti-guinea pig #706-545-148, Cy5 donkey anti-mouse #715-175-511, Cy5 donkey anti-rabbit #711-175-152, Alexa Fluor 647 donkey anti-guinea pig #706-605-148).

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Rabbit polyclonal anti-VIP , Immunostar , Cat# 20077; RRID: AB_572270; LOT# 1513001.

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: For immunostaining, free-floating brain sections were blocked with 5% normal donkey serum in 0.1% Triton X-100 in PBS (PBS-T) for 2 h. Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK-8 (1:1000, Sigma Millipore, C2581, rabbit anti-proCCK (Frontier Institute, Af350), rabbit polyclonal anti-GABA (1:1000, Sigma Millipore, A2052), rabbit polyclonal anti-PV antibody (1:1000, Abcam, ab11427), rabbit polyclonal anti-vasoactive intestinal peptide (VIP) (1:500, Immunostar, 20077), rat polyclonal anti-somatostatin (1:500, Millipore, MAB354), rabbit polyclonal anti-cFos (1:1000, Santa Cruz Biotechnology), chicken polyclonal anti-GFP (1:1000, Abcam, ab13970), and goat polyclonal anti-mCherry (1:1000, Sicgen, AB0040-200).

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: Rabbit polyclonal anti-vasoactive intestinal peptide , Immunostar , Cat# 20077; RRID: AB_572270.

    Article Title: Cholecystokinin-Expressing Interneurons of the Medial Prefrontal Cortex Mediate Working Memory Retrieval
    Article Snippet: Primary antibody incubation was followed by incubation for 2 h at room temperature with PBS-T containing the following secondary antibodies: AlexaFluor-488-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711545152), AlexaFluor-594-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 715515152), Cy5-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711175152), AlexaFluor-488-conjugated donkey anti-chicken (1:1000, Jackson ImmunoResearch Laboratories, 703545145), and AlexaFluor-594-conjugated donkey anti-goat (1:1000, Jackson ImmunoResearch Laboratories, 705515147).

    Plasmid Preparation:

    Article Title: Synaptic Specializations of Melanopsin-Retinal Ganglion Cells in Multiple Brain Regions Revealed by Genetic Label for Light and Electron Microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-miniSOG A gift from Roger Tsien’s laboratory N/A Rabbit polyclonal anti-VIP Immunostar Cat# 20077; RRID: AB_572270 FluoroMyelin Thermo Fisher Scientific Cat# F34652; RRID: AB_2572213 Bacterial and Virus Strains AAV2-EF1a-DIO-miniSOG-f The Salk vector core N/A AAV2-EF1a-DIO-tdTomato-f The Salk vector core N/A Chemicals, Peptides, and Recombinant Proteins Normal donkey serum Jackson ImmunoResearch Cat# 017-000-001 Glutaraldehyde Electron Microscopy Sciences Cat# 16200 Paraformaldehyde Electron Microscopy Sciences Cat# 19200 Glycine Electron Microscopy Sciences Cat# 16200 Potassium cyanide Electron Microscopy Sciences Cat# 19200 Aminotriazole Sigma-Aldrich Cat# G7126 3,30-Diaminobenzidine Free Base Sigma-Aldrich Cat# D8801 Potassium ferrocyanide BAKER ANALYZED A.C.S.

    Article Title: Immunohistochemical Distribution and Neurochemical Characterization of Huntingtin-Associated Protein 1 Immunoreactive Neurons in the Adult Mouse Lingual Ganglia
    Article Snippet: Rabbit Polyclonal anti-VIP , Porcine VIP coupled to bovine thyroglobulin with carbodiimide , Cat# 20077, RRID: AB_572270 , Immunostar, Hudson, WI, USA , 1:5000 , [ ] .

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Fluorophore-conjugated secondary antibodies were from Life Technologies (Alexa Fluor 568 goat anti-rat #A11077, Alexa Fluor 488 donkey anti-mouse #A21202, Alexa Fluor 647 donkey anti-goat #A21447) and Jackson ImmunoResearch (Cy2 donkey anti-goat #705-225-147, Cy2 donkey anti-guinea pig #706-225-148, Cy3 donkey anti-rabbit #711-165-152, Cy3 donkey anti-mouse #715-165-151, Cy3 donkey anti-goat #705-165-147, Alexa Fluor 488 donkey anti-guinea pig #706-545-148, Cy5 donkey anti-mouse #715-175-511, Cy5 donkey anti-rabbit #711-175-152, Alexa Fluor 647 donkey anti-guinea pig #706-605-148).

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Rabbit polyclonal anti-VIP , Immunostar , Cat# 20077; RRID: AB_572270; LOT# 1513001.

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: For immunostaining, free-floating brain sections were blocked with 5% normal donkey serum in 0.1% Triton X-100 in PBS (PBS-T) for 2 h. Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK-8 (1:1000, Sigma Millipore, C2581, rabbit anti-proCCK (Frontier Institute, Af350), rabbit polyclonal anti-GABA (1:1000, Sigma Millipore, A2052), rabbit polyclonal anti-PV antibody (1:1000, Abcam, ab11427), rabbit polyclonal anti-vasoactive intestinal peptide (VIP) (1:500, Immunostar, 20077), rat polyclonal anti-somatostatin (1:500, Millipore, MAB354), rabbit polyclonal anti-cFos (1:1000, Santa Cruz Biotechnology), chicken polyclonal anti-GFP (1:1000, Abcam, ab13970), and goat polyclonal anti-mCherry (1:1000, Sicgen, AB0040-200).

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: Rabbit polyclonal anti-vasoactive intestinal peptide , Immunostar , Cat# 20077; RRID: AB_572270.

    Article Title: Cholecystokinin-Expressing Interneurons of the Medial Prefrontal Cortex Mediate Working Memory Retrieval
    Article Snippet: Primary antibody incubation was followed by incubation for 2 h at room temperature with PBS-T containing the following secondary antibodies: AlexaFluor-488-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711545152), AlexaFluor-594-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 715515152), Cy5-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711175152), AlexaFluor-488-conjugated donkey anti-chicken (1:1000, Jackson ImmunoResearch Laboratories, 703545145), and AlexaFluor-594-conjugated donkey anti-goat (1:1000, Jackson ImmunoResearch Laboratories, 705515147).

    Recombinant:

    Article Title: Synaptic Specializations of Melanopsin-Retinal Ganglion Cells in Multiple Brain Regions Revealed by Genetic Label for Light and Electron Microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-miniSOG A gift from Roger Tsien’s laboratory N/A Rabbit polyclonal anti-VIP Immunostar Cat# 20077; RRID: AB_572270 FluoroMyelin Thermo Fisher Scientific Cat# F34652; RRID: AB_2572213 Bacterial and Virus Strains AAV2-EF1a-DIO-miniSOG-f The Salk vector core N/A AAV2-EF1a-DIO-tdTomato-f The Salk vector core N/A Chemicals, Peptides, and Recombinant Proteins Normal donkey serum Jackson ImmunoResearch Cat# 017-000-001 Glutaraldehyde Electron Microscopy Sciences Cat# 16200 Paraformaldehyde Electron Microscopy Sciences Cat# 19200 Glycine Electron Microscopy Sciences Cat# 16200 Potassium cyanide Electron Microscopy Sciences Cat# 19200 Aminotriazole Sigma-Aldrich Cat# G7126 3,30-Diaminobenzidine Free Base Sigma-Aldrich Cat# D8801 Potassium ferrocyanide BAKER ANALYZED A.C.S.

    Article Title: Immunohistochemical Distribution and Neurochemical Characterization of Huntingtin-Associated Protein 1 Immunoreactive Neurons in the Adult Mouse Lingual Ganglia
    Article Snippet: Rabbit Polyclonal anti-VIP , Porcine VIP coupled to bovine thyroglobulin with carbodiimide , Cat# 20077, RRID: AB_572270 , Immunostar, Hudson, WI, USA , 1:5000 , [ ] .

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Fluorophore-conjugated secondary antibodies were from Life Technologies (Alexa Fluor 568 goat anti-rat #A11077, Alexa Fluor 488 donkey anti-mouse #A21202, Alexa Fluor 647 donkey anti-goat #A21447) and Jackson ImmunoResearch (Cy2 donkey anti-goat #705-225-147, Cy2 donkey anti-guinea pig #706-225-148, Cy3 donkey anti-rabbit #711-165-152, Cy3 donkey anti-mouse #715-165-151, Cy3 donkey anti-goat #705-165-147, Alexa Fluor 488 donkey anti-guinea pig #706-545-148, Cy5 donkey anti-mouse #715-175-511, Cy5 donkey anti-rabbit #711-175-152, Alexa Fluor 647 donkey anti-guinea pig #706-605-148).

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Rabbit polyclonal anti-VIP , Immunostar , Cat# 20077; RRID: AB_572270; LOT# 1513001.

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: For immunostaining, free-floating brain sections were blocked with 5% normal donkey serum in 0.1% Triton X-100 in PBS (PBS-T) for 2 h. Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK-8 (1:1000, Sigma Millipore, C2581, rabbit anti-proCCK (Frontier Institute, Af350), rabbit polyclonal anti-GABA (1:1000, Sigma Millipore, A2052), rabbit polyclonal anti-PV antibody (1:1000, Abcam, ab11427), rabbit polyclonal anti-vasoactive intestinal peptide (VIP) (1:500, Immunostar, 20077), rat polyclonal anti-somatostatin (1:500, Millipore, MAB354), rabbit polyclonal anti-cFos (1:1000, Santa Cruz Biotechnology), chicken polyclonal anti-GFP (1:1000, Abcam, ab13970), and goat polyclonal anti-mCherry (1:1000, Sicgen, AB0040-200).

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: Rabbit polyclonal anti-vasoactive intestinal peptide , Immunostar , Cat# 20077; RRID: AB_572270.

    Article Title: Cholecystokinin-Expressing Interneurons of the Medial Prefrontal Cortex Mediate Working Memory Retrieval
    Article Snippet: Primary antibody incubation was followed by incubation for 2 h at room temperature with PBS-T containing the following secondary antibodies: AlexaFluor-488-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711545152), AlexaFluor-594-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 715515152), Cy5-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711175152), AlexaFluor-488-conjugated donkey anti-chicken (1:1000, Jackson ImmunoResearch Laboratories, 703545145), and AlexaFluor-594-conjugated donkey anti-goat (1:1000, Jackson ImmunoResearch Laboratories, 705515147).

    Software:

    Article Title: Synaptic Specializations of Melanopsin-Retinal Ganglion Cells in Multiple Brain Regions Revealed by Genetic Label for Light and Electron Microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-miniSOG A gift from Roger Tsien’s laboratory N/A Rabbit polyclonal anti-VIP Immunostar Cat# 20077; RRID: AB_572270 FluoroMyelin Thermo Fisher Scientific Cat# F34652; RRID: AB_2572213 Bacterial and Virus Strains AAV2-EF1a-DIO-miniSOG-f The Salk vector core N/A AAV2-EF1a-DIO-tdTomato-f The Salk vector core N/A Chemicals, Peptides, and Recombinant Proteins Normal donkey serum Jackson ImmunoResearch Cat# 017-000-001 Glutaraldehyde Electron Microscopy Sciences Cat# 16200 Paraformaldehyde Electron Microscopy Sciences Cat# 19200 Glycine Electron Microscopy Sciences Cat# 16200 Potassium cyanide Electron Microscopy Sciences Cat# 19200 Aminotriazole Sigma-Aldrich Cat# G7126 3,30-Diaminobenzidine Free Base Sigma-Aldrich Cat# D8801 Potassium ferrocyanide BAKER ANALYZED A.C.S.

    Article Title: Immunohistochemical Distribution and Neurochemical Characterization of Huntingtin-Associated Protein 1 Immunoreactive Neurons in the Adult Mouse Lingual Ganglia
    Article Snippet: Rabbit Polyclonal anti-VIP , Porcine VIP coupled to bovine thyroglobulin with carbodiimide , Cat# 20077, RRID: AB_572270 , Immunostar, Hudson, WI, USA , 1:5000 , [ ] .

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Fluorophore-conjugated secondary antibodies were from Life Technologies (Alexa Fluor 568 goat anti-rat #A11077, Alexa Fluor 488 donkey anti-mouse #A21202, Alexa Fluor 647 donkey anti-goat #A21447) and Jackson ImmunoResearch (Cy2 donkey anti-goat #705-225-147, Cy2 donkey anti-guinea pig #706-225-148, Cy3 donkey anti-rabbit #711-165-152, Cy3 donkey anti-mouse #715-165-151, Cy3 donkey anti-goat #705-165-147, Alexa Fluor 488 donkey anti-guinea pig #706-545-148, Cy5 donkey anti-mouse #715-175-511, Cy5 donkey anti-rabbit #711-175-152, Alexa Fluor 647 donkey anti-guinea pig #706-605-148).

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Rabbit polyclonal anti-VIP , Immunostar , Cat# 20077; RRID: AB_572270; LOT# 1513001.

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: For immunostaining, free-floating brain sections were blocked with 5% normal donkey serum in 0.1% Triton X-100 in PBS (PBS-T) for 2 h. Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK-8 (1:1000, Sigma Millipore, C2581, rabbit anti-proCCK (Frontier Institute, Af350), rabbit polyclonal anti-GABA (1:1000, Sigma Millipore, A2052), rabbit polyclonal anti-PV antibody (1:1000, Abcam, ab11427), rabbit polyclonal anti-vasoactive intestinal peptide (VIP) (1:500, Immunostar, 20077), rat polyclonal anti-somatostatin (1:500, Millipore, MAB354), rabbit polyclonal anti-cFos (1:1000, Santa Cruz Biotechnology), chicken polyclonal anti-GFP (1:1000, Abcam, ab13970), and goat polyclonal anti-mCherry (1:1000, Sicgen, AB0040-200).

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: Rabbit polyclonal anti-vasoactive intestinal peptide , Immunostar , Cat# 20077; RRID: AB_572270.

    Article Title: Cholecystokinin-Expressing Interneurons of the Medial Prefrontal Cortex Mediate Working Memory Retrieval
    Article Snippet: Primary antibody incubation was followed by incubation for 2 h at room temperature with PBS-T containing the following secondary antibodies: AlexaFluor-488-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711545152), AlexaFluor-594-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 715515152), Cy5-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711175152), AlexaFluor-488-conjugated donkey anti-chicken (1:1000, Jackson ImmunoResearch Laboratories, 703545145), and AlexaFluor-594-conjugated donkey anti-goat (1:1000, Jackson ImmunoResearch Laboratories, 705515147).

    Reporter Assay:

    Article Title: Synaptic Specializations of Melanopsin-Retinal Ganglion Cells in Multiple Brain Regions Revealed by Genetic Label for Light and Electron Microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-miniSOG A gift from Roger Tsien’s laboratory N/A Rabbit polyclonal anti-VIP Immunostar Cat# 20077; RRID: AB_572270 FluoroMyelin Thermo Fisher Scientific Cat# F34652; RRID: AB_2572213 Bacterial and Virus Strains AAV2-EF1a-DIO-miniSOG-f The Salk vector core N/A AAV2-EF1a-DIO-tdTomato-f The Salk vector core N/A Chemicals, Peptides, and Recombinant Proteins Normal donkey serum Jackson ImmunoResearch Cat# 017-000-001 Glutaraldehyde Electron Microscopy Sciences Cat# 16200 Paraformaldehyde Electron Microscopy Sciences Cat# 19200 Glycine Electron Microscopy Sciences Cat# 16200 Potassium cyanide Electron Microscopy Sciences Cat# 19200 Aminotriazole Sigma-Aldrich Cat# G7126 3,30-Diaminobenzidine Free Base Sigma-Aldrich Cat# D8801 Potassium ferrocyanide BAKER ANALYZED A.C.S.

    Article Title: Immunohistochemical Distribution and Neurochemical Characterization of Huntingtin-Associated Protein 1 Immunoreactive Neurons in the Adult Mouse Lingual Ganglia
    Article Snippet: Rabbit Polyclonal anti-VIP , Porcine VIP coupled to bovine thyroglobulin with carbodiimide , Cat# 20077, RRID: AB_572270 , Immunostar, Hudson, WI, USA , 1:5000 , [ ] .

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Fluorophore-conjugated secondary antibodies were from Life Technologies (Alexa Fluor 568 goat anti-rat #A11077, Alexa Fluor 488 donkey anti-mouse #A21202, Alexa Fluor 647 donkey anti-goat #A21447) and Jackson ImmunoResearch (Cy2 donkey anti-goat #705-225-147, Cy2 donkey anti-guinea pig #706-225-148, Cy3 donkey anti-rabbit #711-165-152, Cy3 donkey anti-mouse #715-165-151, Cy3 donkey anti-goat #705-165-147, Alexa Fluor 488 donkey anti-guinea pig #706-545-148, Cy5 donkey anti-mouse #715-175-511, Cy5 donkey anti-rabbit #711-175-152, Alexa Fluor 647 donkey anti-guinea pig #706-605-148).

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Rabbit polyclonal anti-VIP , Immunostar , Cat# 20077; RRID: AB_572270; LOT# 1513001.

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: For immunostaining, free-floating brain sections were blocked with 5% normal donkey serum in 0.1% Triton X-100 in PBS (PBS-T) for 2 h. Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK-8 (1:1000, Sigma Millipore, C2581, rabbit anti-proCCK (Frontier Institute, Af350), rabbit polyclonal anti-GABA (1:1000, Sigma Millipore, A2052), rabbit polyclonal anti-PV antibody (1:1000, Abcam, ab11427), rabbit polyclonal anti-vasoactive intestinal peptide (VIP) (1:500, Immunostar, 20077), rat polyclonal anti-somatostatin (1:500, Millipore, MAB354), rabbit polyclonal anti-cFos (1:1000, Santa Cruz Biotechnology), chicken polyclonal anti-GFP (1:1000, Abcam, ab13970), and goat polyclonal anti-mCherry (1:1000, Sicgen, AB0040-200).

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: Rabbit polyclonal anti-vasoactive intestinal peptide , Immunostar , Cat# 20077; RRID: AB_572270.

    Article Title: Cholecystokinin-Expressing Interneurons of the Medial Prefrontal Cortex Mediate Working Memory Retrieval
    Article Snippet: Primary antibody incubation was followed by incubation for 2 h at room temperature with PBS-T containing the following secondary antibodies: AlexaFluor-488-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711545152), AlexaFluor-594-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 715515152), Cy5-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711175152), AlexaFluor-488-conjugated donkey anti-chicken (1:1000, Jackson ImmunoResearch Laboratories, 703545145), and AlexaFluor-594-conjugated donkey anti-goat (1:1000, Jackson ImmunoResearch Laboratories, 705515147).

    RNAscope:

    Article Title: Synaptic Specializations of Melanopsin-Retinal Ganglion Cells in Multiple Brain Regions Revealed by Genetic Label for Light and Electron Microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-miniSOG A gift from Roger Tsien’s laboratory N/A Rabbit polyclonal anti-VIP Immunostar Cat# 20077; RRID: AB_572270 FluoroMyelin Thermo Fisher Scientific Cat# F34652; RRID: AB_2572213 Bacterial and Virus Strains AAV2-EF1a-DIO-miniSOG-f The Salk vector core N/A AAV2-EF1a-DIO-tdTomato-f The Salk vector core N/A Chemicals, Peptides, and Recombinant Proteins Normal donkey serum Jackson ImmunoResearch Cat# 017-000-001 Glutaraldehyde Electron Microscopy Sciences Cat# 16200 Paraformaldehyde Electron Microscopy Sciences Cat# 19200 Glycine Electron Microscopy Sciences Cat# 16200 Potassium cyanide Electron Microscopy Sciences Cat# 19200 Aminotriazole Sigma-Aldrich Cat# G7126 3,30-Diaminobenzidine Free Base Sigma-Aldrich Cat# D8801 Potassium ferrocyanide BAKER ANALYZED A.C.S.

    Article Title: Immunohistochemical Distribution and Neurochemical Characterization of Huntingtin-Associated Protein 1 Immunoreactive Neurons in the Adult Mouse Lingual Ganglia
    Article Snippet: Rabbit Polyclonal anti-VIP , Porcine VIP coupled to bovine thyroglobulin with carbodiimide , Cat# 20077, RRID: AB_572270 , Immunostar, Hudson, WI, USA , 1:5000 , [ ] .

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Fluorophore-conjugated secondary antibodies were from Life Technologies (Alexa Fluor 568 goat anti-rat #A11077, Alexa Fluor 488 donkey anti-mouse #A21202, Alexa Fluor 647 donkey anti-goat #A21447) and Jackson ImmunoResearch (Cy2 donkey anti-goat #705-225-147, Cy2 donkey anti-guinea pig #706-225-148, Cy3 donkey anti-rabbit #711-165-152, Cy3 donkey anti-mouse #715-165-151, Cy3 donkey anti-goat #705-165-147, Alexa Fluor 488 donkey anti-guinea pig #706-545-148, Cy5 donkey anti-mouse #715-175-511, Cy5 donkey anti-rabbit #711-175-152, Alexa Fluor 647 donkey anti-guinea pig #706-605-148).

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Rabbit polyclonal anti-VIP , Immunostar , Cat# 20077; RRID: AB_572270; LOT# 1513001.

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: For immunostaining, free-floating brain sections were blocked with 5% normal donkey serum in 0.1% Triton X-100 in PBS (PBS-T) for 2 h. Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK-8 (1:1000, Sigma Millipore, C2581, rabbit anti-proCCK (Frontier Institute, Af350), rabbit polyclonal anti-GABA (1:1000, Sigma Millipore, A2052), rabbit polyclonal anti-PV antibody (1:1000, Abcam, ab11427), rabbit polyclonal anti-vasoactive intestinal peptide (VIP) (1:500, Immunostar, 20077), rat polyclonal anti-somatostatin (1:500, Millipore, MAB354), rabbit polyclonal anti-cFos (1:1000, Santa Cruz Biotechnology), chicken polyclonal anti-GFP (1:1000, Abcam, ab13970), and goat polyclonal anti-mCherry (1:1000, Sicgen, AB0040-200).

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: Rabbit polyclonal anti-vasoactive intestinal peptide , Immunostar , Cat# 20077; RRID: AB_572270.

    Article Title: Cholecystokinin-Expressing Interneurons of the Medial Prefrontal Cortex Mediate Working Memory Retrieval
    Article Snippet: Primary antibody incubation was followed by incubation for 2 h at room temperature with PBS-T containing the following secondary antibodies: AlexaFluor-488-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711545152), AlexaFluor-594-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 715515152), Cy5-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711175152), AlexaFluor-488-conjugated donkey anti-chicken (1:1000, Jackson ImmunoResearch Laboratories, 703545145), and AlexaFluor-594-conjugated donkey anti-goat (1:1000, Jackson ImmunoResearch Laboratories, 705515147).

    Multiplex Assay:

    Article Title: Synaptic Specializations of Melanopsin-Retinal Ganglion Cells in Multiple Brain Regions Revealed by Genetic Label for Light and Electron Microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-miniSOG A gift from Roger Tsien’s laboratory N/A Rabbit polyclonal anti-VIP Immunostar Cat# 20077; RRID: AB_572270 FluoroMyelin Thermo Fisher Scientific Cat# F34652; RRID: AB_2572213 Bacterial and Virus Strains AAV2-EF1a-DIO-miniSOG-f The Salk vector core N/A AAV2-EF1a-DIO-tdTomato-f The Salk vector core N/A Chemicals, Peptides, and Recombinant Proteins Normal donkey serum Jackson ImmunoResearch Cat# 017-000-001 Glutaraldehyde Electron Microscopy Sciences Cat# 16200 Paraformaldehyde Electron Microscopy Sciences Cat# 19200 Glycine Electron Microscopy Sciences Cat# 16200 Potassium cyanide Electron Microscopy Sciences Cat# 19200 Aminotriazole Sigma-Aldrich Cat# G7126 3,30-Diaminobenzidine Free Base Sigma-Aldrich Cat# D8801 Potassium ferrocyanide BAKER ANALYZED A.C.S.

    Article Title: Immunohistochemical Distribution and Neurochemical Characterization of Huntingtin-Associated Protein 1 Immunoreactive Neurons in the Adult Mouse Lingual Ganglia
    Article Snippet: Rabbit Polyclonal anti-VIP , Porcine VIP coupled to bovine thyroglobulin with carbodiimide , Cat# 20077, RRID: AB_572270 , Immunostar, Hudson, WI, USA , 1:5000 , [ ] .

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Fluorophore-conjugated secondary antibodies were from Life Technologies (Alexa Fluor 568 goat anti-rat #A11077, Alexa Fluor 488 donkey anti-mouse #A21202, Alexa Fluor 647 donkey anti-goat #A21447) and Jackson ImmunoResearch (Cy2 donkey anti-goat #705-225-147, Cy2 donkey anti-guinea pig #706-225-148, Cy3 donkey anti-rabbit #711-165-152, Cy3 donkey anti-mouse #715-165-151, Cy3 donkey anti-goat #705-165-147, Alexa Fluor 488 donkey anti-guinea pig #706-545-148, Cy5 donkey anti-mouse #715-175-511, Cy5 donkey anti-rabbit #711-175-152, Alexa Fluor 647 donkey anti-guinea pig #706-605-148).

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Rabbit polyclonal anti-VIP , Immunostar , Cat# 20077; RRID: AB_572270; LOT# 1513001.

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: For immunostaining, free-floating brain sections were blocked with 5% normal donkey serum in 0.1% Triton X-100 in PBS (PBS-T) for 2 h. Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK-8 (1:1000, Sigma Millipore, C2581, rabbit anti-proCCK (Frontier Institute, Af350), rabbit polyclonal anti-GABA (1:1000, Sigma Millipore, A2052), rabbit polyclonal anti-PV antibody (1:1000, Abcam, ab11427), rabbit polyclonal anti-vasoactive intestinal peptide (VIP) (1:500, Immunostar, 20077), rat polyclonal anti-somatostatin (1:500, Millipore, MAB354), rabbit polyclonal anti-cFos (1:1000, Santa Cruz Biotechnology), chicken polyclonal anti-GFP (1:1000, Abcam, ab13970), and goat polyclonal anti-mCherry (1:1000, Sicgen, AB0040-200).

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: Rabbit polyclonal anti-vasoactive intestinal peptide , Immunostar , Cat# 20077; RRID: AB_572270.

    Article Title: Cholecystokinin-Expressing Interneurons of the Medial Prefrontal Cortex Mediate Working Memory Retrieval
    Article Snippet: Primary antibody incubation was followed by incubation for 2 h at room temperature with PBS-T containing the following secondary antibodies: AlexaFluor-488-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711545152), AlexaFluor-594-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 715515152), Cy5-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711175152), AlexaFluor-488-conjugated donkey anti-chicken (1:1000, Jackson ImmunoResearch Laboratories, 703545145), and AlexaFluor-594-conjugated donkey anti-goat (1:1000, Jackson ImmunoResearch Laboratories, 705515147).

    Targeted Proteomics:

    Article Title: Synaptic Specializations of Melanopsin-Retinal Ganglion Cells in Multiple Brain Regions Revealed by Genetic Label for Light and Electron Microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-miniSOG A gift from Roger Tsien’s laboratory N/A Rabbit polyclonal anti-VIP Immunostar Cat# 20077; RRID: AB_572270 FluoroMyelin Thermo Fisher Scientific Cat# F34652; RRID: AB_2572213 Bacterial and Virus Strains AAV2-EF1a-DIO-miniSOG-f The Salk vector core N/A AAV2-EF1a-DIO-tdTomato-f The Salk vector core N/A Chemicals, Peptides, and Recombinant Proteins Normal donkey serum Jackson ImmunoResearch Cat# 017-000-001 Glutaraldehyde Electron Microscopy Sciences Cat# 16200 Paraformaldehyde Electron Microscopy Sciences Cat# 19200 Glycine Electron Microscopy Sciences Cat# 16200 Potassium cyanide Electron Microscopy Sciences Cat# 19200 Aminotriazole Sigma-Aldrich Cat# G7126 3,30-Diaminobenzidine Free Base Sigma-Aldrich Cat# D8801 Potassium ferrocyanide BAKER ANALYZED A.C.S.

    Article Title: Immunohistochemical Distribution and Neurochemical Characterization of Huntingtin-Associated Protein 1 Immunoreactive Neurons in the Adult Mouse Lingual Ganglia
    Article Snippet: Rabbit Polyclonal anti-VIP , Porcine VIP coupled to bovine thyroglobulin with carbodiimide , Cat# 20077, RRID: AB_572270 , Immunostar, Hudson, WI, USA , 1:5000 , [ ] .

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Fluorophore-conjugated secondary antibodies were from Life Technologies (Alexa Fluor 568 goat anti-rat #A11077, Alexa Fluor 488 donkey anti-mouse #A21202, Alexa Fluor 647 donkey anti-goat #A21447) and Jackson ImmunoResearch (Cy2 donkey anti-goat #705-225-147, Cy2 donkey anti-guinea pig #706-225-148, Cy3 donkey anti-rabbit #711-165-152, Cy3 donkey anti-mouse #715-165-151, Cy3 donkey anti-goat #705-165-147, Alexa Fluor 488 donkey anti-guinea pig #706-545-148, Cy5 donkey anti-mouse #715-175-511, Cy5 donkey anti-rabbit #711-175-152, Alexa Fluor 647 donkey anti-guinea pig #706-605-148).

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Rabbit polyclonal anti-VIP , Immunostar , Cat# 20077; RRID: AB_572270; LOT# 1513001.

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: For immunostaining, free-floating brain sections were blocked with 5% normal donkey serum in 0.1% Triton X-100 in PBS (PBS-T) for 2 h. Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK-8 (1:1000, Sigma Millipore, C2581, rabbit anti-proCCK (Frontier Institute, Af350), rabbit polyclonal anti-GABA (1:1000, Sigma Millipore, A2052), rabbit polyclonal anti-PV antibody (1:1000, Abcam, ab11427), rabbit polyclonal anti-vasoactive intestinal peptide (VIP) (1:500, Immunostar, 20077), rat polyclonal anti-somatostatin (1:500, Millipore, MAB354), rabbit polyclonal anti-cFos (1:1000, Santa Cruz Biotechnology), chicken polyclonal anti-GFP (1:1000, Abcam, ab13970), and goat polyclonal anti-mCherry (1:1000, Sicgen, AB0040-200).

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: Rabbit polyclonal anti-vasoactive intestinal peptide , Immunostar , Cat# 20077; RRID: AB_572270.

    Article Title: Cholecystokinin-Expressing Interneurons of the Medial Prefrontal Cortex Mediate Working Memory Retrieval
    Article Snippet: Primary antibody incubation was followed by incubation for 2 h at room temperature with PBS-T containing the following secondary antibodies: AlexaFluor-488-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711545152), AlexaFluor-594-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 715515152), Cy5-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711175152), AlexaFluor-488-conjugated donkey anti-chicken (1:1000, Jackson ImmunoResearch Laboratories, 703545145), and AlexaFluor-594-conjugated donkey anti-goat (1:1000, Jackson ImmunoResearch Laboratories, 705515147).

    Microscopy:

    Article Title: Synaptic Specializations of Melanopsin-Retinal Ganglion Cells in Multiple Brain Regions Revealed by Genetic Label for Light and Electron Microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-miniSOG A gift from Roger Tsien’s laboratory N/A Rabbit polyclonal anti-VIP Immunostar Cat# 20077; RRID: AB_572270 FluoroMyelin Thermo Fisher Scientific Cat# F34652; RRID: AB_2572213 Bacterial and Virus Strains AAV2-EF1a-DIO-miniSOG-f The Salk vector core N/A AAV2-EF1a-DIO-tdTomato-f The Salk vector core N/A Chemicals, Peptides, and Recombinant Proteins Normal donkey serum Jackson ImmunoResearch Cat# 017-000-001 Glutaraldehyde Electron Microscopy Sciences Cat# 16200 Paraformaldehyde Electron Microscopy Sciences Cat# 19200 Glycine Electron Microscopy Sciences Cat# 16200 Potassium cyanide Electron Microscopy Sciences Cat# 19200 Aminotriazole Sigma-Aldrich Cat# G7126 3,30-Diaminobenzidine Free Base Sigma-Aldrich Cat# D8801 Potassium ferrocyanide BAKER ANALYZED A.C.S.

    Article Title: Immunohistochemical Distribution and Neurochemical Characterization of Huntingtin-Associated Protein 1 Immunoreactive Neurons in the Adult Mouse Lingual Ganglia
    Article Snippet: Rabbit Polyclonal anti-VIP , Porcine VIP coupled to bovine thyroglobulin with carbodiimide , Cat# 20077, RRID: AB_572270 , Immunostar, Hudson, WI, USA , 1:5000 , [ ] .

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Fluorophore-conjugated secondary antibodies were from Life Technologies (Alexa Fluor 568 goat anti-rat #A11077, Alexa Fluor 488 donkey anti-mouse #A21202, Alexa Fluor 647 donkey anti-goat #A21447) and Jackson ImmunoResearch (Cy2 donkey anti-goat #705-225-147, Cy2 donkey anti-guinea pig #706-225-148, Cy3 donkey anti-rabbit #711-165-152, Cy3 donkey anti-mouse #715-165-151, Cy3 donkey anti-goat #705-165-147, Alexa Fluor 488 donkey anti-guinea pig #706-545-148, Cy5 donkey anti-mouse #715-175-511, Cy5 donkey anti-rabbit #711-175-152, Alexa Fluor 647 donkey anti-guinea pig #706-605-148).

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Rabbit polyclonal anti-VIP , Immunostar , Cat# 20077; RRID: AB_572270; LOT# 1513001.

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: For immunostaining, free-floating brain sections were blocked with 5% normal donkey serum in 0.1% Triton X-100 in PBS (PBS-T) for 2 h. Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK-8 (1:1000, Sigma Millipore, C2581, rabbit anti-proCCK (Frontier Institute, Af350), rabbit polyclonal anti-GABA (1:1000, Sigma Millipore, A2052), rabbit polyclonal anti-PV antibody (1:1000, Abcam, ab11427), rabbit polyclonal anti-vasoactive intestinal peptide (VIP) (1:500, Immunostar, 20077), rat polyclonal anti-somatostatin (1:500, Millipore, MAB354), rabbit polyclonal anti-cFos (1:1000, Santa Cruz Biotechnology), chicken polyclonal anti-GFP (1:1000, Abcam, ab13970), and goat polyclonal anti-mCherry (1:1000, Sicgen, AB0040-200).

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: Rabbit polyclonal anti-vasoactive intestinal peptide , Immunostar , Cat# 20077; RRID: AB_572270.

    Article Title: Cholecystokinin-Expressing Interneurons of the Medial Prefrontal Cortex Mediate Working Memory Retrieval
    Article Snippet: Primary antibody incubation was followed by incubation for 2 h at room temperature with PBS-T containing the following secondary antibodies: AlexaFluor-488-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711545152), AlexaFluor-594-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 715515152), Cy5-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711175152), AlexaFluor-488-conjugated donkey anti-chicken (1:1000, Jackson ImmunoResearch Laboratories, 703545145), and AlexaFluor-594-conjugated donkey anti-goat (1:1000, Jackson ImmunoResearch Laboratories, 705515147).

    Electron Microscopy:

    Article Title: Synaptic Specializations of Melanopsin-Retinal Ganglion Cells in Multiple Brain Regions Revealed by Genetic Label for Light and Electron Microscopy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-miniSOG A gift from Roger Tsien’s laboratory N/A Rabbit polyclonal anti-VIP Immunostar Cat# 20077; RRID: AB_572270 FluoroMyelin Thermo Fisher Scientific Cat# F34652; RRID: AB_2572213 Bacterial and Virus Strains AAV2-EF1a-DIO-miniSOG-f The Salk vector core N/A AAV2-EF1a-DIO-tdTomato-f The Salk vector core N/A Chemicals, Peptides, and Recombinant Proteins Normal donkey serum Jackson ImmunoResearch Cat# 017-000-001 Glutaraldehyde Electron Microscopy Sciences Cat# 16200 Paraformaldehyde Electron Microscopy Sciences Cat# 19200 Glycine Electron Microscopy Sciences Cat# 16200 Potassium cyanide Electron Microscopy Sciences Cat# 19200 Aminotriazole Sigma-Aldrich Cat# G7126 3,30-Diaminobenzidine Free Base Sigma-Aldrich Cat# D8801 Potassium ferrocyanide BAKER ANALYZED A.C.S.

    Article Title: Immunohistochemical Distribution and Neurochemical Characterization of Huntingtin-Associated Protein 1 Immunoreactive Neurons in the Adult Mouse Lingual Ganglia
    Article Snippet: Rabbit Polyclonal anti-VIP , Porcine VIP coupled to bovine thyroglobulin with carbodiimide , Cat# 20077, RRID: AB_572270 , Immunostar, Hudson, WI, USA , 1:5000 , [ ] .

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Fluorophore-conjugated secondary antibodies were from Life Technologies (Alexa Fluor 568 goat anti-rat #A11077, Alexa Fluor 488 donkey anti-mouse #A21202, Alexa Fluor 647 donkey anti-goat #A21447) and Jackson ImmunoResearch (Cy2 donkey anti-goat #705-225-147, Cy2 donkey anti-guinea pig #706-225-148, Cy3 donkey anti-rabbit #711-165-152, Cy3 donkey anti-mouse #715-165-151, Cy3 donkey anti-goat #705-165-147, Alexa Fluor 488 donkey anti-guinea pig #706-545-148, Cy5 donkey anti-mouse #715-175-511, Cy5 donkey anti-rabbit #711-175-152, Alexa Fluor 647 donkey anti-guinea pig #706-605-148).

    Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
    Article Snippet: Rabbit polyclonal anti-VIP , Immunostar , Cat# 20077; RRID: AB_572270; LOT# 1513001.

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: For immunostaining, free-floating brain sections were blocked with 5% normal donkey serum in 0.1% Triton X-100 in PBS (PBS-T) for 2 h. Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK-8 (1:1000, Sigma Millipore, C2581, rabbit anti-proCCK (Frontier Institute, Af350), rabbit polyclonal anti-GABA (1:1000, Sigma Millipore, A2052), rabbit polyclonal anti-PV antibody (1:1000, Abcam, ab11427), rabbit polyclonal anti-vasoactive intestinal peptide (VIP) (1:500, Immunostar, 20077), rat polyclonal anti-somatostatin (1:500, Millipore, MAB354), rabbit polyclonal anti-cFos (1:1000, Santa Cruz Biotechnology), chicken polyclonal anti-GFP (1:1000, Abcam, ab13970), and goat polyclonal anti-mCherry (1:1000, Sicgen, AB0040-200).

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
    Article Snippet: Rabbit polyclonal anti-vasoactive intestinal peptide , Immunostar , Cat# 20077; RRID: AB_572270.

    Article Title: Cholecystokinin-Expressing Interneurons of the Medial Prefrontal Cortex Mediate Working Memory Retrieval
    Article Snippet: Primary antibody incubation was followed by incubation for 2 h at room temperature with PBS-T containing the following secondary antibodies: AlexaFluor-488-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711545152), AlexaFluor-594-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 715515152), Cy5-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711175152), AlexaFluor-488-conjugated donkey anti-chicken (1:1000, Jackson ImmunoResearch Laboratories, 703545145), and AlexaFluor-594-conjugated donkey anti-goat (1:1000, Jackson ImmunoResearch Laboratories, 705515147).



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    93
    Bio-Rad rabbit polyclonal anti vip
    Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of <t>VPAC1</t> and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) <t>type</t> <t>1</t> receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.
    Rabbit Polyclonal Anti Vip, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ImmunoStar inc rabbit polyclonal anti-vasoactive intestinal peptide (vip)
    Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of <t>VPAC1</t> and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) <t>type</t> <t>1</t> receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.
    Rabbit Polyclonal Anti Vasoactive Intestinal Peptide (Vip), supplied by ImmunoStar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology polyclonal rabbit anti-vasoactive intestinal peptide (vip) sc-20727
    Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of <t>VPAC1</t> and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) <t>type</t> <t>1</t> receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.
    Polyclonal Rabbit Anti Vasoactive Intestinal Peptide (Vip) Sc 20727, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Yanaihara Institute Inc rabbit polyclonal anti-human vasoactive intestinal peptide (vip)
    Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of <t>VPAC1</t> and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) <t>type</t> <t>1</t> receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.
    Rabbit Polyclonal Anti Human Vasoactive Intestinal Peptide (Vip), supplied by Yanaihara Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Journal: iScience

    Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory

    doi: 10.1016/j.isci.2024.108824

    Figure Lengend Snippet:

    Article Snippet: For immunostaining, free-floating brain sections were blocked with 5% normal donkey serum in 0.1% Triton X-100 in PBS (PBS-T) for 2 h. Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK-8 (1:1000, Sigma Millipore, C2581, rabbit anti-proCCK (Frontier Institute, Af350), rabbit polyclonal anti-GABA (1:1000, Sigma Millipore, A2052), rabbit polyclonal anti-PV antibody (1:1000, Abcam, ab11427), rabbit polyclonal anti-vasoactive intestinal peptide (VIP) (1:500, Immunostar, 20077), rat polyclonal anti-somatostatin (1:500, Millipore, MAB354), rabbit polyclonal anti-cFos (1:1000, Santa Cruz Biotechnology), chicken polyclonal anti-GFP (1:1000, Abcam, ab13970), and goat polyclonal anti-mCherry (1:1000, Sicgen, AB0040-200).

    Techniques: Virus, Plasmid Preparation, Recombinant, Software

    Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of VPAC1 and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) type 1 receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.

    Journal: Biology

    Article Title: Alterations in Gut Microbiota and Upregulations of VPAC2 and Intestinal Tight Junctions Correlate with Anti-Inflammatory Effects of Electroacupuncture in Colitis Mice with Sleep Fragmentation

    doi: 10.3390/biology11070962

    Figure Lengend Snippet: Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of VPAC1 and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) type 1 receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.

    Article Snippet: In addition, the role of the vasoactive intestinal peptide (VIP) type 1 receptor (VIPR1/VPAC1, CSB-PA052529, Cusabio, Houston, TX, USA) and type 2 receptor (VIPR2/VPAC2, A03768, Boster, Pleasanton, CA, USA) under EA were clarified by immunohistochemical staining.

    Techniques: Immunohistochemical staining, Immunohistochemistry, Expressing, Western Blot